Grapevine testing web order form

Company name:(Required)

Contact person:(Required)

Email:(Required)

Contact phone(Required)

Customer order #:

Affinity Labs quote # (if applicable)

Will these samples be delivered to a regional laboratory or drop-off point?

Affinity Labs has a regional laboratory in the Murray-Darling region (Merbein) and also uses regional partners to help facilitate the movement of customer samples to our Adelaide laboratory. For more information on this free service, contact customer service.

Will these samples be delivered to a regional drop-off point?(Required)

Is the plant material coming from South Australia?(Required)

South Australia

Outside South Australia

Is the plant material coming from a phylloxera control area?(Required)

Phylloxera Infested Zone (PIZ)

Phylloxera Interim Buffer Zone (PIBZ)

Phylloxera Risk Zone (PRZ)

Phylloxera Exclusion Zone (PEZ)

For samples coming from South Australia, the following treatment and documentation is required:

Samples should be refrigerated unless they are likely to take more than three days to reach the laboratory, in which case they should be frozen.
Include a copy of your order confirmation with your samples. No further documentation is required.

Samples collected from within a Phylloxera Infested Zone (PIZ), Phylloxera Interim Buffer Zone (PIBZ) or a Phylloxera Risk Zone (PRZ) must follow procedure C in the National Phylloxera Management Protocol. Treatment and documentation required are:

Samples must undergo disinfestation by a procedure that involves freezing at -18C for at least 24 hours, prior to packaging on cold packs for transport. (Note that dry ice is considered a hazard by some transport companies.)
A permit for Phylloxera Control Areas is required to move grape material out of these zones. A copy of the certificate and permit must be sent with the grapes. A permit application form for Host Material movement into or from a Phylloxera Control area is available from the relevant State Department of Agriculture/Primary Industries. Samples from a PIZ, PIBZ or PRZ shipped without relevant documentation cannot be processed and will be destroyed.
A Plant Material Movement Declaration Form must be completed and shipped with the sample. This is a requirement of the CA12 accreditation granted by Primary Industries and Resources of South Australia (PIRSA) that allows the importation of grapevine material by Affinity Labs for testing purposes.
Include a copy of your order confirmation with your samples.

Samples collected from within a Phylloxera Exclusion Zone (PEZ) require the following treatment and documentation:

A Plant Material Movement Declaration Form must be completed and shipped with the sample. This is a requirement of the CA12 accreditation granted by Primary Industries and Resources of South Australia (PIRSA) that allows the importation of grapevine material by Affinity Labs for testing purposes.
Include a copy of your order confirmation with your samples.

How many samples do you want to submit?

Each sample submitted on this form must be of the same sample type. If you wish to submit multiple sample types for analysis, a new form is needed for each type. All requested tests will be performed on each listed sample. The sample descriptions entered below will appear on your report.

If you have more than 20 samples, please list them in a spreadsheet and email to customerservice@affinitylabs.com.au

Number of samples

Sample 1 description(Required)

Variety clone rootstock

Region

Sample 2 description(Required)

Variety clone rootstock

Region

Sample 3 description(Required)

Variety clone rootstock

Region

Sample 4 description(Required)

Variety clone rootstock

Region

Sample 5 description(Required)

Variety clone rootstock

Region

Sample 6 description(Required)

Variety clone rootstock

Region

Sample 7 description(Required)

Variety clone rootstock

Region

Sample 8 description(Required)

Variety clone rootstock

Region

Sample 9 description(Required)

Variety clone rootstock

Region

Sample 10 description(Required)

Variety clone rootstock

Region

Sample 11 description(Required)

Variety clone rootstock

Region

Sample 12 description(Required)

Variety clone rootstock

Region

Sample 13 description(Required)

Variety clone rootstock

Region

Sample 14 description(Required)

Variety clone rootstock

Region

Sample 15 description(Required)

Variety clone rootstock

Region

Sample 16 description(Required)

Variety clone rootstock

Region

Sample 17 description(Required)

Variety clone rootstock

Region

Sample 18 description(Required)

Variety clone rootstock

Region

Sample 19 description(Required)

Variety clone rootstock

Region

Sample 20 description(Required)

Variety clone rootstock

Region

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Reason for testing:

Top working

Nursery material

Virus status

Checking after virus elimination

Other

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Other - specify

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Plant tissue testing

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Nutrient testing

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Plant tissue testing End

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Virus testing

Each sample submitted for virus testing will be ordered together with a virus sample preparation (TNA extraction) analysis.

PCR virus tests

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Grapevine leafroll associated virus

Grapevine leafroll associated virus 1 (LR1)

Grapevine leafroll associated virus 2 (LR2)

Grapevine leafroll associated virus 3 (LR3)

Grapevine leafroll associated virus 4 (LR4)

Grapevine leafroll associated virus 4 subgroup 6 (LR4/6)

Grapevine leafroll associated virus 4 subgroup 9 (LR4/9)

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Wine plant health web order form

Grapevine rupestris stem pitting-associated virus (GRSPaV)

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Grapevine fleck virus (GFkV)

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Grapevine Pinot Gris virus (GPGV)

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Grapevine virus A (GVA)

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Grapevine virus B (GVB)

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"Grapevine red blotch virus Currently exotic to Australia and requires mandatory reporting if a positive is obtained."

Grapevine red blotch virus (GRBV)
Currently exotic to Australia and requires mandatory reporting if a positive is obtained.

Virus testing packages

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Pre-grafting virus screen (includes 3 viruses)

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Pre-grafting virus screen (includes 4 viruses)

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Complete virus screen (includes 11 viruses)

Methods and limitations of virus testing

Results provided by the AWRI T/A Affinity Labs are STRICTLY LIMITED to assays of ACTUAL SAMPLES provided by the client and to the time of sampling. The client must not assume that the same results apply to any plant other than the specific plants actually sampled at a certain time. Clients should not represent or imply that the results apply other than to the actual samples provided for assay. The client releases and indemnifies the AWRI in respect of any loss or liability incurred as a consequence of any such representations, which remain at all times the sole responsibility of the client.
The PCR diagnostic method used by The AWRI will detect the presence of specific disease agents ONLY above a certain threshold level.
The failure to detect a specific disease agent does not guarantee the complete absence of that disease agent from the sample analysed. In any diagnostic assay, there is a lower limit of detection of a disease agent below which that agent cannot be detected by the above-mentioned techniques used by the AWRI.
Whilst a negative assay result can mean the complete absence of the disease agent, the disease agent may, in some cases, be present at such a low level that it cannot be detected. In any such plants, the disease agent may eventually multiply to a much higher level, at which it can then be detected, and symptoms of infection may appear.
As a consequence, the AWRI cannot guarantee that a sample producing a negative assay result will be completely free of the disease agents being tested for on behalf of the customer. Therefore the client assumes all risk and liability.
Clients should use the assay results as an indicator only of the presence of the disease agent but should not rely upon the results as conclusive of the complete absence of the disease agent in the sample provided. The AWRI assumes no liability.
It is also important to note that the PCR diagnostic assay requires two short DNA primers that are specific for each of the viruses and for the phytoplasmas assayed. The sequence of these DNA primers is determined by the nucleotide sequence of the genetic material being tested.
The AWRI makes clear to the client that there may be strains of one or more of these pathogens that cannot be detected with the specific DNA primers used for the PCR assay.

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Virus testing Section End

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Virus elimination

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Virus elimination

Grapevine virus elimination

Fees and payment

List price: $4,200 per sample (excl. GST) for one virus-free plant returned plus $1,050 (excl GST) for each additional plant requested from that sample.

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How many plants would you like returned to you?(Required)

Payment schedule

50% Up-front payment prior to commencement of virus elimination work

25% After treatment in tissue culture is complete and virus removal is confirmed by PCR (approximately 6 - 12 months after submission)

25% After treated and virus-free plantlets are provided back to the client (approximately 12 - 24 months after submission)

Methods and limitations of virus elimination

One hardened-off plantlet from each virus-eliminated variety will be provided to the client unless extra plants are requested at additional costs (see Fees and payment).

Due to the limit of detection, very low virus titres in treated plantlets may not be detected. It is therefore important that plants returned to clients are retested after two dormancies to confirm their virus-free status. The virus elimination process is not complete until after this final test. If a positive test is returned at this stage, for viruses present in the initial sample, then virus elimination will need to be repeated (at no additional cost). Tissue culture material will be maintained until this final virus test.

Grapevine rupestris stem pitting-associated virus (GRSPaV) is present in almost all grape varieties. We do not guarantee the removal of this virus. Once the plants are established in the field, GRSPaV is detectable by PCR in symptomless plants.

Since mealybugs and scale insects are the vectors of most grapevine viruses, these may re-infect young vines in the vineyard. Annual testing of mother blocks is recommended.

No guarantee is made of elimination of viruses outside of the complete virus screen list (see Grapevine virus testing fact sheet). All virus-eliminated material is tested using the standard screens of the Affinity Labs virus testing service. If a virus outside of these screens is of concern, please contact the customer service team for further information and options.

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Virus elimination Section End

Grapevine variety identification

Grapevine ID

Grapevine variety ID

Methods and limitations of grapevine variety testing

Affinity Labs offers a grapevine variety ID service which involves DNA typing of grapevine material using a single nucleotide polymorphism (SNP) panel made up of 48 SNP markers and comparison to the CSIRO grapevine variety SNP database. This service is provided using data developed and owned by the CSIRO and Wine Australia.

The CSIRO grapevine variety SNP database contains profiles of approximately 360 Vitis vinifera cultivars used for wine-grape, table grape and dried grape production. The database cannot be used to identify non-vinifera species or hybrids between V. vinifera and non-vinifera species (i.e. most rootstocks). There are varying levels of confidence around the SNP profiles and their associated variety names within the CSIRO database. Names have been assigned to one of the below groups based on this level of confidence:

Prime name – A Prime name has been given to a variety in the CSIRO database when its SNP pattern matched an international database genotype that has at least two independent sources of evidence to support that varieties identification. This is the highest level of confidence that a variety name can be given. However, it is important to note that no absolute warranty is given by international databases that such evidence is correct nor independent and hence there is no absolute warranty assigned to any variety name.

Prime global name - In some cases the Prime name is a global name for a cluster of varieties derived from a single original embryo. These varieties cannot be distinguished from one another using SNP profiling. For example, the global Prime name ‘Pinot’ denotes a genotype representing Pinot Noir, Pinot Blanc, Pinot Meunier, Pinot Gris etc.

Provisional name- This is the likely name of the variety however provisional names cannot be taken as the correct name for any genotype. The SNP profile obtained matches a variety that has been assigned a provisional name in the CSIRO database which occurs in two circumstances:

The Australian reference variety most closely matches that of the named variety in an international reference database but is not a complete match, or
The Australian reference variety completely matches a variety in an international database, but in that international database it was assigned a provisional name due to lack of sufficient evidence to fully support the naming.

Sample delivery and quarantine consideration

Leaf material is the preferable tissue to be submitted for this service (two to three young leaves); however, cane samples can also be accepted if required.

To submit samples from outside of South Australia, plant material must be accompanied by a completed plant material movement declaration form and be packaged as detailed in that document. The appropriate declaration form will be provided when you complete this sample submission form and answer the plant material origin and phylloxera control area questions above.

Please contact our customer service team for more information regarding samples being shipped internationally, or samples shipped from a Phylloxera risk or infested zone.

Methods and limitations

This service cannot be used to identify non-vinifera species or hybrids between V. vinifera and non-vinifera species (i.e. most rootstocks).
If the SNP pattern of the submitted grapevine sample does not match one of the varieties in the CSIRO database, it will be reported as ‘unknown’.
No absolute warranty is given to any variety name within the CSIRO grapevine variety SNP database.

Grapevine variety identification

Comments

Please review the AWRI's terms and conditions and privacy policy.

hereby acknowledge(Required)

I hereby acknowledge that the person submitting this form is an authorised representative of the company. I have read and agree to the terms and conditions.

Regional drop-off point acknowledgement(Required)

I agree that by using a regional drop-off point, my submitted information will be disclosed to the collection partner for the purpose of facilitating the delivery of samples to Affinity Labs.

Grapevine testing web order form